Intended use:
- for laboratory use only
- clean up of sample extracts for further analysis
Storage:
Columns should be stored at room temperature in a dry place (preferably sealed in the original packaging protected from direct light).
Description of test method for aflatoxin
Extraction solvent (84+16 acetonitrile + water) is spiked with standard to a final concentration of 10ng/mL.
| HPLC column: | Hitachi LaChrom, C18,4.6×150mm, | Instrument: | HPLC FLD, Hitachi |
| Injection volume: | 20µL | Flow rate: | 1mL/min |
| Excitation/Emission: | 365nm/445nm | KRC25-25: | 254nm |
Description of test method for zearalenone
Extraction solvent (84+16 acetonitrile + water) is spiked with standard to a final concentration of 250ng/mL.
| HPLC column: | Hitachi LaChrom, 4.6×15cm, C18 | Instrument: | HPLC FLD, Hitachi |
| Injection volume: | 20µL | Flow rate: | 1mL/min |
| Excitation: | 274nm/440nm | Emission: | 440nm |
Certified values and their uncertainties:
Columns were analyzed according to the above referenced method and the following criteria were met:
- Percent recovery of aflatoxin and zearalenone on neat spikes:85%~115%
- Naturally contaminated material:±2 standard deviations from the established mean
- %RSD<10%
Intended use:
- for laboratory use only
- clean up of sample extracts for further analysis
Storage:
Columns should be stored at room temperature in a dry place (preferably sealed in the original packaging protected from direct light).
Description of test method for aflatoxin
Extraction solvent (84+16 acetonitrile + water) is spiked with standard to a final concentration of 10ng/mL.
| HPLC column: | Hitachi LaChrom, C18,4.6×150mm, | Instrument: | HPLC FLD, Hitachi |
| Injection volume: | 20µL | Flow rate: | 1mL/min |
| Excitation/Emission: | 365nm/445nm | KRC25-25: | 254nm |
Description of test method for zearalenone
Extraction solvent (84+16 acetonitrile + water) is spiked with standard to a final concentration of 250ng/mL.
| HPLC column: | Hitachi LaChrom, 4.6×15cm, C18 | Instrument: | HPLC FLD, Hitachi |
| Injection volume: | 20µL | Flow rate: | 1mL/min |
| Excitation: | 274nm/440nm | Emission: | 440nm |
Certified values and their uncertainties:
Columns were analyzed according to the above referenced method and the following criteria were met:
- Percent recovery of aflatoxin and zearalenone on neat spikes:85%~115%
- Naturally contaminated material:±2 standard deviations from the established mean
- %RSD<10%
Chromatogram obtained with test method:


Special notes for column use:
à The packing in the columns may shift or settle during the shipping process. If this occurs, before using the column, push the frit firmly onto the packing material using a blunt object such as an unsharpened wooden pencil.
à After transferring the sample into the culture tube, place the column firmly into the top portion of the tube. You may tilt thetube slightly to wet the sides of the culture tube with the sample extract. This makes it easier to push the column into the tube.
à PriboFast®columns are designed for single use only. Any improper use or reuse of the columns may affect the reliability of the results.
à Culture tubes used for most PriboFast® columns are 15 mm X 85 mm.
à Analytical methodology using Pribolab products that has been developed and validated by Pribolab.
is available by request. Validation of other methodology using Pribolab products is the responsibility of the user. Pribolab Analytical Services are available by request to assist in validation of other methodologies.
PriboFast® Storage and Expiration:
When stored in a cool, dry place (preferably sealed in the original packaging protected from direct light), Pribolab guarantees the quality of the PriboFast® columns up to 30 months after date of manufacture. PriboFast® columns used after this time period must be validated by the user to ensure adequate performance. Exclusion of Warranty Statement
The user assumes all risk in using PriboFast® products and services. Pribolab will warrant that its products and services meet all quality control standards set by Pribolab, and Pribolab will, at its option, rapair or replace any product, components, or repeat services which prove to be defective in workmanship or material within product specific warranty periods or expiration dates and which our examination shall disclose to our satisfaction to be defective as such. This warranty is expressly in lieu of all other warranties, expressed or implied, as to description, quality, merchantability, fitness for any particular purpose, productiveness or any other matter. Pribolab shall be in no way responsible for the proper use of its products. Pribolab here by disclaims all other remedies, warranties, guarantees or liabilities, expressed or implied, arising by law or otherwise, and it shall have no liability for any lost profits or damage, direct, indirect or otherwise, to person or property, in connection with the use of any of its products or services. This warranty shall not be extended, altered or varies except by a written instrument signed by an authorized representative of Pribolab.
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