Pribolab - PRS-014-50黄曲霉毒素B1荧光定量快速检测卡-50-42531

Product Introduction

Aflatoxin B1 (Aflatoxin B1, AFB 1) is a metabolite produced by some Aspergillus (such as Aspergillus and

Aspergillus parasitic), which is strongly carcinogenic and extremely toxic. Aflatoxin has a destructive effect on

human and animal liver tissue, and can lead to liver cancer and even death in severe cases. There are four main

types of aflatoxin in naturally contaminated food: B1, B2, G1 and G2, among which aflatoxin B1 is the most

common and the most toxic and carcinogenic.

This product is used to quickly detect the residual amount of aflatoxin B1 in grain, feed, vegetable oil, etc. The

sample preprocessing is simple, and the testing process should only be about 12 min (5 min + 7 min). It is suitable

for all kinds of grain, food and feed processing enterprises, grain depots, third-party testing agencies and

government regulatory authorities.

Detection principle

This product is based on the time-resolved fluorescence immunochromatography rapid quantitative detection

technology platform. When the solution to the test to the aflatoxin B1 rapid fluorescence quantitative detection

card (hereinafter referred to as the "detection card"), the aflatoxin B1 to the fluorescent microsphere labeled

aflatoxin B1 antibody of the binding pad in the combination and reached the capillary, the aflatoxin B1 antigen

fixed on the detection line T to the remaining unbound fluorescent microsphere labeled aflatoxin B1 antibody.

After the reaction, the fluorescence intensity of T line and C line are read by the fluorescence detector

(hereinafter referred to as "detector") and calculate the T / C value, calculate the content of aflatoxin B1 in the

sample and determine the Yin and Yang.

Product Performance Index

Curve range: 1-50 ppb

Sample extract solution was prepared

(1) 50% ethanol water extract: take 500 mL of pure water, add 500 mL absolute ethanol (AR grade), mix and seal

at room temperature.

(2) 70% ethanol water extract: take 300 mL of pure water, add 700 mL absolute ethanol (AR grade), mix and seal

at room temperature.

(3) 30% acetonitrile water extract: take 700 mL of pure water, add 300 mL of acetonitrile (AR grade), mix and seal

at room temperature.

Note: ① laboratories without water production conditions can be purchased to use Wahaha pure water; ②

prepared volume can be proportional amplification.

Preparation before testing

1. Please read the operation instructions carefully before the experiment, and return the waiting test card,

sample extract and sample dilutions to the temperature for more than 30 min at room temperature (25℃);

2. Turn on the constant temperature incubator of the test card and use it after the temperature rises to 37℃;

3. Turn on the detector, preheat for 10 min, insert the ID card of this batch, and select the corresponding curve.

Sample preprocessing

1. sample preparation

Solid, such as grain feed、peanut meal:

Take 300-500 g of samples to be tested, and crush the particles to 90% to pass 20 mesh sieve;

Vegetable oil and peanut buttersamples:

① Vegetable oil sample: take more than 20 g of representative crude oil, centrifuge at 5000 r/min for 2 min, and

sample the upper liquid after centrifugation (weigh the vegetable oil directly except for crude oil, without

centrifugation);

② Peanut butter: samples should be fully mixed evenly, and representative samples should be taken;

2. sample treatment

(1) Accurately weigh 5.0 g ± 0.01 g sample and place it in a 50 mL centrifuge tube.

(2) 25 mL of sample extraction solution was added, mixed for 3 minutes and centrifuged at 5000 rpm for 2

minutes at room temperature.

(3) Take 100 μL of supernatant in a new centrifuge tube, add 500 μ L of sample dilution, mix well (vortex for 7

seconds, or hand for 1 minute) as the sample to be tested.

Note:

Part of the strong absorbent sample take 2.5 ± 0.025 g for extraction, the rest of the operation is the same, the

test result by 2 is the final concentration.

Detection steps

1. Remove the test card from the aluminum foil bag and place it horizontally on the test card thermostatic

incubator (please use it as soon as possible within 10 min);

2. Draw 100 μ L of the diluted solution to be tested with a pipette gun and add it to the sample well on the test

card, and start the timing lid incubation for 7 min.

3. Insert the data card into the corresponding socket of the detector, and click the "Quick Detection" key on the

detector screen to enter the detection program. Select the standard curve corresponding to the sample to

be tested.

4. After the incubation, immediately insert the end of the detection card facing forward (handheld end facing

outward) into the detector, and click the "Quick Detection" button on the screen for detection (please

strictly control the reaction time for 7min).

5. Read / print the test results from the tester.

result output

1. The test results will be displayed on the detector screen, and you can press the print button to obtain the

paper test report, or by exporting the computer;

2. Invalid: the test may be invalid due to the expired test card, non-standard operation and other reasons, you

need to find out the reason and re-test;

3. When the test result is beyond the curve range maximum, and further dilution is required. In the feed

samples as an example,take 1 mL of centrifugation of supernatant in centrifuge tube and dilute with 50%

ethanol solution (e. g., 5 times dilution: take 100μL of centrifugal supernatant in centrifuge tube + 400μL of

50% ethanol solution) and then mix 100 uL of mixture + 500 uL of diluent for testing. The reading result of

the instrument shall be multiplied by the corresponding dilution multiple.

matters need attention

1. Test cards should be used within the validity period. Test cards, ID cards and diluents of different batches

and different items should not be mixed;

2. After the completion of the experiment, the test results will be checked in the "history record" of thedetector interface.

3. Please follow the operation steps and do not touch the strip scanning area during the operation.

4. Please pay attention to the consistency of the experimental operation. Centrigation immediately after the

vortex oscillation. After the centrifugation, remove the supernatant immediately to avoid the extraction

effect over time.

5. Do not blow the detector and incubator through the air conditioning outlet.

6. This strip is a disposable product, do not reuse it.

7. After 7 min of incubation, please conduct the test immediately (complete all test cards within 2 min after

incubation);

8. This method is only used for primary screening. In case of positive samples, please use the confirmation

method for confirmation.

9. For any problems encountered in the experiment, please contact the supplier.

Storage conditions and shelf-life

Storage conditions: keep the detection card in 2~8℃, in a cool, dark and dry environment.

Shelf-life: see the outer packaging.