1. General information
This document is designed and the certified value(s) and uncertainty(ies) are determined in accordance with ISO Guide 31[1].
2.1 Intended use of the RM
- for laboratory use only
- calibration of analytical instruments。
2.2 Instruction for the correct use of the RM
The compound should be stored at 2-8℃ in a dark place. Before usage of the RM , the compound should be allowed to warm to temperature(20±3℃). The recommended minimum sub - sample amount for all kinds of application is 100 μL. The expiry date of this RM is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages.
2.3 Hazardous situation
The normal laboratory safety precautions should be observed when working with this RM.Further details for the handing of this RM are available as safety data sheet.
Hazardous Ingredients Concentration in% Pictograms Signal word Hazard statement(s)
Acetonitrile >99.9 
Danger H225,H302,H312,H319,H332
3. Certified values and their uncertainties
| Deoxynivalenolin acetonitrile | ||
| Compound | Mass concentration a | |
| Certified valueb | Uncertaintyc | |
| Deoxynivalenol | 100.2μg/mL | ±0.8μg/mL |
| a Mass concentration based on weighed amount ,purity and dilution steps | b Values are based on preparation data and confirmed experimentally by HPLC-DAD | c Expanded uncertainty U(k=2) of the value uc according to GUM[2] |
3.1 Calculation of uncertainty
After the concentration of the gravimetric prepared solution was confirmed by HPLC-DAD,the uncertainty of the calibrant was calculated on the basis of preparation[3].
| Uncertainty components | Description | Standard uncertainty(U) |
| Purity(P)of solid Deoxynivalenol | P=99.5±0.5% | u(P)=0.3% (a) |
| Weighing procedure Weighted sample: mws=20.141mg | U(m)=0.0000008g+1.30*10-5*m Toxinu(m)=U(m)/2 | u(m)=0.0005mg(b) |
| Dilution procedure Volumetric flask :Vf=200mL | Calibration:200mL ± 0.15mL Repeatability : 0.03mL Volume expansion solvent | u(cal)=0.06mL(c) u(rep)=0.03mL(d) u(Vol.exp.1)=0.47mL(e) u(V)=0.47mL (f) |
a Maximum tolerance of purity was divided by
b Calculation of this u-value is based upon the uncertainty formula for the weighed amount as given in the calibration report from annual balance calibration
c A triangular distribution(division by )was chosen for the calculation of u(cal)
d Based on a series of ten fill and weigh experiments on a typical 200mL flask; the value was used directly as a standard deviation
e Based on the density of 0.7857 g/cm3 at temperature T=20℃ and a maximum temperature variation of ±3℃,of volume expansion, relative volume expansion coefficient of acetonitrile is 1370*10-6/℃[4],volume expansion term(rectangular distribution)was divided by
f The three contributions are combined to give the u(V)=
Calculation of the combined uncertainty u c and the expanded standard uncertainty U
calculation of expanded standard uncertainty U using a coverage factor k=2
4.Discussion of traceability
This calibrant is certified on the basis of gravimetric preparation[3]. Thus the certified value(mass concentration of Deoxynivalenolis based on the weighed amount of the starting material and is therefore traceable to the stated purity of the solid raw material. High purity material represents a practical realization of concentration units, through conversion of mass to molar quantity.
5. Confirmation of certified value by HPLC-DAD
The certified concentration of Deoxynivalenol of the gravimetric prepared solution was confirmed by HPLC-DAD against an independently prepared reference batch of Deoxynivalenol.
6.Further information
The purchaser must determine the suitability of this product for its particular use. Pribolab makes no warranty of any kind, express or implied, other than its products meet all quality control standards set by Pribolab. We do not guarantee that the product can be used for a special application.
Inspected by 
Quality System Specialist
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