Pribolab - STD#7072Pribolab®交链孢霉属毒素混合标准品/甲醇AOH 6ppm、AME 0.6ppm、TEN 3ppm，TEA 15 ppm-96412
Pribolab®交链孢霉属毒素混合标准品/甲醇AOH 6ppm、AME 0.6ppm、TEN 3ppm，TEA 15 ppm
1. General Information
This document is designed and the certified value(s) and uncertainty(ies) are determined in accordance with ISO Guide 31.^[1]^
2.1 Intended Use of the RM
- For laboratory use only
- Calibration of analytical instruments
2.2 Instruction for Correct Use
The compound should be stored at 2-8℃ or below in a dark place. Before usage of the RM,the compound should be allowed to warm to temperature(20±3℃). The recommended minimum sub - sample amount for all kinds of application is 100 μL. The expiry date of this RM is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages.
2.3 Hazardous Situation
| Ingredient | Concentration | Signal word | Hazard statements |
|---|---|---|---|
| Methanol | >99.9% | ⚠ Danger | H225,H301,H311,H331,H370 |
3. Certified values and their uncertainties
| Alternariol,Alternariol monomethyl ether,Tenuazonic acid,Tentoxin in Methanol | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Compound | Mass concentration a | Compound | Mass concentration a | ||||||||
| Certified valueb | Uncertaintyc | Certified valueb | Uncertaintyc | ||||||||
| Alternariol | 6.00μg/mL | ±0.072μg/mL | Alternariol monomethyl ether | 0.604μg/mL | ±0.004μg/mL | ||||||
| Tentoxin | 3.00μg/mL | ±0.04μg/mL | Tenuazonic acid | 15.00μg/mL | ±0.24μg/mL | ||||||
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3. Calculation of uncertainty
After the concentration of the gravimetric prepared solution was confirmed by HPLC-DAD,the uncertainty of the calibrant was calculated on the basis of preparation[3].
| Uncertainty components | Description | Standard uncertainty/uc |
|---|---|---|
|
Purity(P) of solid Alternariol Alternariol monomethyl ether Tentoxin Tenuazonic acid |
P1=99.0± 1.0% P2=99.5 ± 0.5% P3=98.8 ± 1.2% P4=98.7 ± 1.3% |
u(P1)=0.6%↓ u(P2)=0.3% u(P3)=0.7% u(P4)=0.8% |
|
Weighing procedure: Weighted sample mAlternariol=3.030mg mAlternariol monomethyl ether=0.304mg mTentoxin =1.518mg mTenuazonic acid=7.599mg |
U(m)=0.0000008g+1.30*10-5*mToxin u(m)=U(m)/2 |
u1m=0.0004mg |
|
Dilution procedure Volumetric flask1:Vf1=500mL±0.25mL repeatability : 0.1mL |
u(cal1)=0.1mL u(rep1)=0.1mL u(Vol.exp.1)=1.03mL u(V1)=1.04mL |
a Maximum tolerance of purity was divided by √3
b Calculation of this u-value is based upon the uncertainty formula for the weighed amount as given in the calibration report from annual balance calibration
c A triangular distribution(division by √6 ) was chosen for the calculation of u(cal)a
d Based on a series of ten fill and weigh experiments on a typical 250mL flask; the value was used directly as a standard deviation
e Based on the density of 0.7918 g/cm3 at temperature T=20℃ and a maximum temperature variation of ±3℃,of volume expansion, relative volume expansion coefficient of acetonitrile is 1190*10-6/℃[4], volume expansion term(rectangular distribution) was divided by √3
f,i,m The three contributions are combined to give the u(V)=√[u(cal)2+u(rep)2+u(Vol.exp)2]
Calculation of the combined uncertainty uc and the expanded standard uncertainty U
CToxin = 10 × mws × P1 × Vp / Vf1 × Vf2 = 10 × 3.030 × 99.0 / 500 = 6.00 mg / L ↓
uc(CToxin) = √[ (u(P1)/P1)2 + (u(m)/mws)2 + (u(Vf1)/Vf1)2 + (u(Vf2)/Vf2)2 + (u(Vp)/Vp)2 ] = √[ (0.6/99.0)2 + (0.0004/3.030)2 + (1.04/500)2 ] = 0.006
uc(CToxin) = CToxin × 0.006 = 6.00 × 0.006 = 0.036 mg / L
calculation of expanded standard uncertainty U using a coverage factor k=2
U(CToxin) = uc(CToxin) × 2 = 0.036 × 2 = 0.072 mg / L = 0.072μg / mL ↓
4. Discussion of Traceability
This calibrant is certified on the basis of gravimetric preparation[3]. Thus the certified value(mass concentration of Multi-Toxin is based on the weighed amount of the starting material and is therefore traceable to the stated purity of the solid raw material. High purity material represents a practical realization of concentration units, through conversion of mass to molar quantity.
5. Confirmation by HPLC-DAD
The certified concentration of Multi-Toxin of the gravimetric prepared solution was confirmed by HPLC-DAD against an independently prepared reference batch of Multi-Toxin.
6. Further Information
The purchaser must determine the suitability of this product for its particular use. Pribolab makes no warranty of any kind, express or implied, other than its products meet all quality control standards set by Pribolab. We do not guarantee that the product can be used for a special application.
Inspected by 
Quality System Specialist
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